Project description:Cells were grown at 30oC in YPD medium (buffered to pH 5.0 with 7.5 mM succinate) containing ET solution to a density of 0.6 X 107 cells/ml (A600 0.6). The culture was supplemented with ET at 30oC for 15 min. Cells were collected for the t=0 min sample. Next, an equal volume of YPD medium (pH 5.0) containing ET and 0.4M CaCl2 was added to the ET-treated culture (final concentration 0.2 M CaCl2). Cells were collected by centrifugation at 5, 15, 30 and 60 min, frozen at 80oC and processed for RNA extraction. For the publication, the experiment was repeated twoce, and corresponding timepoints from each experiment were averaged. The first 4 and last 4 arrays in this set correspond to each experiment.

2005-01-01 | E-SMDB-7 | biostudies-arrayexpress

Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.

Project description: Not available

| S-EPMC5555045 | biostudies-literature