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Cryo-ET of cryosections of S. pombe cells in G1 arrest and in G0 quiescence


ABSTRACT:

SUBMITTER: Lu Gan 

PROVIDER: EMPIAR-10339 | biostudies-other |

REPOSITORIES: biostudies-other

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Macromolecular and cytological changes in fission yeast G0 nuclei.

Tan Zhi Yang ZY   Cai 蔡舒君 Shujun S   Paithankar Saayli A SA   Liu Tingsheng T   Nie Xin X   Shi Jian J   Gan 甘露 Lu L  

Journal of cell science 20250327 6


When starved of nitrogen, cells of the fission yeast Schizosaccharomyces pombe enter a quiescent 'G0' state with smaller nuclei and transcriptional repression. The genomics of S. pombe G0 cells has been well studied, but much of its nuclear cell biology remains unknown. Here, we use confocal microscopy, immunoblots and electron cryotomography to investigate the cytological, biochemical and ultrastructural differences between S. pombe proliferating, G1-arrested and G0 cell nuclei, with an emphasi  ...[more]

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2015-01-27 | GSE61088 | GEO