Dataset Information
Single-Particle Cryo-EM of Arabinosyltransferase EmbB from Mycobacterium smegmatis, Collected using both Falcon III and K2 Detectors
Ontology highlight
ABSTRACT:
SUBMITTER: Yong Zi Tan
PROVIDER: EMPIAR-10420 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.
Nature communications 20200707 1
Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting ...[more]