Movies of DPS in 260 nm gold foil holes, which eliminate specimen movement
Ontology highlight
ABSTRACT:
SUBMITTER: Christopher John Russo
PROVIDER: EMPIAR-10445 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM with sub-1 Å specimen movement.
Naydenova Katerina K Jia Peipei P Russo Christopher J CJ
Science (New York, N.Y.) 20201001 6513
Most information loss in cryogenic electron microscopy (cryo-EM) stems from particle movement during imaging, which remains poorly understood. We show that this movement is caused by buckling and subsequent deformation of the suspended ice, with a threshold that depends directly on the shape of the frozen water layer set by the support foil. We describe a specimen support design that eliminates buckling and reduces electron beam-induced particle movement to less than 1 angstrom. The design allow ...[more]
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