Dataset Information
Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles at subnanometer resolution
Ontology highlight
ABSTRACT:
SUBMITTER: Misha Kudryashev
PROVIDER: EMPIAR-10452 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM.
Nature communications 20200724 1
Cryo-electron tomography combined with subtomogram averaging (StA) has yielded high-resolution structures of macromolecules in their native context. However, high-resolution StA is not commonplace due to beam-induced sample drift, images with poor signal-to-noise ratios (SNR), challenges in CTF correction, and limited particle number. Here we address these issues by collecting tilt series with a higher electron dose at the zero-degree tilt. Particles of interest are then located within reconstru ...[more]