Dataset Information
ISWI-NCP complex in the ADP-bound state
Ontology highlight
ABSTRACT:
SUBMITTER: Zhucheng Chen
PROVIDER: EMPIAR-10483 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structures of the ISWI-nucleosome complex reveal a conserved mechanism of chromatin remodeling.
Nature structural & molecular biology 20190313 4
Chromatin remodelers are diverse enzymes, and different models have been proposed to explain how these proteins work. Here we report the 3.3 Å-resolution cryogenic electron microscopy (cryo-EM) structures of Saccharomyces cerevisiae ISWI (ISW1) in complex with the nucleosome in adenosine diphosphate (ADP)-bound and ADP-BeF<sub>x</sub>-bound states. The data show that after nucleosome binding, ISW1 is activated by substantial rearrangement of the catalytic domains, with the regulatory AutoN domai ...[more]