Dataset Information
Serial cryoFIB/SEM reveals cytoarchitectural disruptions in Leigh syndrome patient cells
Ontology highlight
ABSTRACT:
SUBMITTER: Peijun Zhang
PROVIDER: EMPIAR-10515 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells.
Structure (London, England : 1993) 20201022 1
The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutation in US ...[more]
