Dataset Information
Cryo-EM dataset of the substrate-engaged human 26S proteasome
Ontology highlight
ABSTRACT:
SUBMITTER: Youdong Jack Mao
PROVIDER: EMPIAR-10669 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome.
Nature 20181112 7737
The proteasome is an ATP-dependent, 2.5-megadalton molecular machine that is responsible for selective protein degradation in eukaryotic cells. Here we present cryo-electron microscopy structures of the substrate-engaged human proteasome in seven conformational states at 2.8-3.6 Å resolution, captured during breakdown of a polyubiquitylated protein. These structures illuminate a spatiotemporal continuum of dynamic substrate-proteasome interactions from ubiquitin recognition to substrate transloc ...[more]