Yeast C, Ci, C*, and P complex spliceosomes
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ABSTRACT:
SUBMITTER: Max Wilkinson
PROVIDER: EMPIAR-10687 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis for conformational equilibrium of the catalytic spliceosome.
Wilkinson Max E ME Fica Sebastian M SM Galej Wojciech P WP Nagai Kiyoshi K
Molecular cell 20210310 7
The ATPase Prp16 governs equilibrium between the branching (B<sup>∗</sup>/C) and exon ligation (C<sup>∗</sup>/P) conformations of the spliceosome. Here, we present the electron cryomicroscopy reconstruction of the Saccharomyces cerevisiae C-complex spliceosome at 2.8 Å resolution and identify a novel C-complex intermediate (C<sub>i</sub>) that elucidates the molecular basis for this equilibrium. The exon-ligation factors Prp18 and Slu7 bind to C<sub>i</sub> before ATP hydrolysis by Prp16 can des ...[more]
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