Dataset Information
Single particle Data of diazaborine bound Drg1
Ontology highlight
ABSTRACT:
SUBMITTER: David Haselbach
PROVIDER: EMPIAR-10717 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.
Nature communications 20210609 1
The hexameric AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis and initiates cytoplasmic maturation of the large ribosomal subunit by releasing the shuttling maturation factor Rlp24. Drg1 monomers contain two AAA-domains (D1 and D2) that act in a concerted manner. Rlp24 release is inhibited by the drug diazaborine which blocks ATP hydrolysis in D2. The mode of inhibition was unknown. Here we show the first cryo-EM structure of Drg1 revealing the inhibitory mechanism. Diazaborine ...[more]