Dataset Information
Cryo-EM structure of SARS-CoV-2 spike at pH 4.0
Ontology highlight
ABSTRACT:
SUBMITTER: Sergei Pletnev
PROVIDER: EMPIAR-10723 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains.
Cell host & microbe 20201117 6
The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the human ACE2 receptor and to facilitate virus entry, which can occur through low-pH-endosomal pathways. To understand how ACE2 binding and low pH affect spike conformation, we determined cryo-electron microscopy structures-at serological and endosomal pH-delineating spike recognition of up to three ACE2 molecules. RBDs freely adopted "up" conformations required for ACE2 interaction, primarily through RBD movement com ...[more]