Dataset Information
Tilt series of SARS-CoV-2 spike-bearing virus-like particles (VLPs) interacting with hACE2-bearing extracellular vesicles (tEVs), showing various intermediate states of the SARS-CoV-2 spike protein
Ontology highlight
ABSTRACT:
SUBMITTER: Tara Charlie Marcink
PROVIDER: EMPIAR-10788 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Intermediates in SARS-CoV-2 spike-mediated cell entry.
Science advances 20220819 33
SARS-CoV-2 cell entry is completed after viral spike (S) protein-mediated membrane fusion between viral and host cell membranes. Stable prefusion and postfusion S structures have been resolved by cryo-electron microscopy and cryo-electron tomography, but the refolding intermediates on the fusion pathway are transient and have not been examined. We used an antiviral lipopeptide entry inhibitor to arrest S protein refolding and thereby capture intermediates as S proteins interact with hACE2 and fu ...[more]