Dataset Information
Conformational rearrangements upon start codon recognition in human 48S translation initiation complex
Ontology highlight
ABSTRACT:
SUBMITTER: Niels Fischer
PROVIDER: EMPIAR-11005 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Conformational rearrangements upon start codon recognition in human 48S translation initiation complex.
Nucleic acids research 20220501 9
Selection of the translation start codon is a key step during protein synthesis in human cells. We obtained cryo-EM structures of human 48S initiation complexes and characterized the intermediates of codon recognition by kinetic methods using eIF1A as a reporter. Both approaches capture two distinct ribosome populations formed on an mRNA with a cognate AUG codon in the presence of eIF1, eIF1A, eIF2-GTP-Met-tRNAiMet and eIF3. The 'open' 40S subunit conformation differs from the human 48S scanning ...[more]
