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Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex

ABSTRACT:

SUBMITTER: Tsukasa Kusakizako

PROVIDER: EMPIAR-11099 | biostudies-other |

REPOSITORIES: biostudies-other

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Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.

Yamaguchi Sonomi S Naganuma Masahiro M Nishizawa Tomohiro T Kusakizako Tsukasa T Tomari Yukihide Y Nishimasu Hiroshi H Nureki Osamu O

Nature 20220629 7918

In flies, Argonaute2 (Ago2) and small interfering RNA (siRNA) form an RNA-induced silencing complex to repress viral transcripts<sup>1</sup>. The RNase III enzyme Dicer-2 associates with its partner protein R2D2 and cleaves long double-stranded RNAs to produce 21-nucleotide siRNA duplexes, which are then loaded into Ago2 in a defined orientation<sup>2-5</sup>. Here we report cryo-electron microscopy structures of the Dicer-2-R2D2 and Dicer-2-R2D2-siRNA complexes. R2D2 interacts with the helicase ...[more]

PMID: 35768503

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