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Cryo electron tomography of H. neapolitanus alpha-carboxysomes


ABSTRACT:

SUBMITTER: Grant J Jensen 

PROVIDER: EMPIAR-11125 | biostudies-other |

REPOSITORIES: biostudies-other

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Despite the importance of microcompartments in prokaryotic biology and bioengineering, structural heterogeneity has prevented a complete understanding of their architecture, ultrastructure, and spatial organization. Here, we employ cryo-electron tomography to image α-carboxysomes, a pseudo-icosahedral microcompartment responsible for carbon fixation. We have solved a high-resolution subtomogram average of the Rubisco cargo inside the carboxysome, and determined the arrangement of the enzyme. We  ...[more]

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