Dataset Information
A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed-refractory multiple myeloma
Ontology highlight
ABSTRACT:
SUBMITTER: Alwin Krämer
PROVIDER: EMPIAR-11243 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed/refractory multiple myeloma.
Cell reports methods 20221101 11
Electron microscopy is the gold standard to characterize centrosomal ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. We therefore developed a generalizable, semi-automated high-throughput electron tomography strategy to study centrosome aberrations in sparse patient-derived cancer cells at nanoscale. As proof of principle, we present electron tomography data on 455 centrioles of CD138<sup>pos</sup> plasma cells from one patient with r ...[more]