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Cryo-electron tomography on plasma FIB lamellae of HeLa cells

ABSTRACT:

SUBMITTER: Michael Grange

PROVIDER: EMPIAR-11306 | biostudies-other |

REPOSITORIES: biostudies-other

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Plasma FIB milling for the determination of structures in situ.

Berger Casper C Dumoux Maud M Glen Thomas T Yee Neville B-Y NB Mitchels John M JM Patáková Zuzana Z Darrow Michele C MC Naismith James H JH Grange Michael M

Nature communications 20230206 1

Structural biology studies inside cells and tissues require methods to thin vitrified specimens to electron transparency. Until now, focused ion beams based on gallium have been used. However, ion implantation, changes to surface chemistry and an inability to access high currents limit gallium application. Here, we show that plasma-coupled ion sources can produce cryogenic lamellae of vitrified human cells in a robust and automated manner, with quality sufficient for pseudo-atomic structure dete ...[more]

PMID: 36746945

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