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Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, donor DNA, and target DNA

ABSTRACT:

SUBMITTER: Hiroshi Nishimasu

PROVIDER: EMPIAR-11804 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural mechanism of bridge RNA-guided recombination.

Hiraizumi Masahiro M Perry Nicholas T NT Durrant Matthew G MG Soma Teppei T Nagahata Naoto N Okazaki Sae S Athukoralage Januka S JS Isayama Yukari Y Pai James J JJ Pawluk April A Konermann Silvana S Yamashita Keitaro K Hsu Patrick D PD Nishimasu Hiroshi H

Nature 20240626 8018

Insertion sequence (IS) elements are the simplest autonomous transposable elements found in prokaryotic genomes<sup>1</sup>. We recently discovered that IS110 family elements encode a recombinase and a non-coding bridge RNA (bRNA) that confers modular specificity for target DNA and donor DNA through two programmable loops<sup>2</sup>. Here we report the cryo-electron microscopy structures of the IS110 recombinase in complex with its bRNA, target DNA and donor DNA in three different stages of the ...[more]

PMID: 38926616

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