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Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex

ABSTRACT:

SUBMITTER: Hiroshi Nishimasu

PROVIDER: EMPIAR-11825 | biostudies-other |

REPOSITORIES: biostudies-other

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Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex.

Kato Kazuki K Zhou Wenyuan W Okazaki Sae S Isayama Yukari Y Nishizawa Tomohiro T Gootenberg Jonathan S JS Abudayyeh Omar O OO Nishimasu Hiroshi H

Cell 20220527 13

The type III-E CRISPR-Cas effector Cas7-11, with dual RNase activities for precursor CRISPR RNA (pre-crRNA) processing and crRNA-guided target RNA cleavage, is a new platform for bacterial and mammalian RNA targeting. We report the 2.5-Å resolution cryoelectron microscopy structure of Cas7-11 in complex with a crRNA and its target RNA. Cas7-11 adopts a modular architecture comprising seven domains (Cas7.1-Cas7.4, Cas11, INS, and CTE) and four interdomain linkers. The crRNA 5' tag is recognized a ...[more]

PMID: 35643083

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