Dataset Information
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2H-nucleosome complex without crosslinking
Ontology highlight
ABSTRACT:
SUBMITTER: Jean-Paul Armache
PROVIDER: EMPIAR-11909 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
Nature communications 20240312 1
Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI). Here, to address this issue, we develop graphene-oxide-coated EM grids functionalized with either single-stranded DNA (ssDNA) or thiol-poly(acrylic acid-co-styrene) (TAASTY) co-polymer. These grids protect complexes between the chromatin remodeler SNF2h and nucleosomes from ...[more]