Cryo electron microscopy of in vitro assembled NC-4 cages
Ontology highlight
ABSTRACT:
SUBMITTER: Stephan Tetter
PROVIDER: EMPIAR-11973 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Stimulus-responsive assembly of nonviral nucleocapsids.
Hori Mao M Steinauer Angela A Tetter Stephan S Hälg Jamiro J Manz Eva-Maria EM Hilvert Donald D
Nature communications 20240427 1
Controlled assembly of a protein shell around a viral genome is a key step in the life cycle of many viruses. Here we report a strategy for regulating the co-assembly of nonviral proteins and nucleic acids into highly ordered nucleocapsids in vitro. By fusing maltose binding protein to the subunits of NC-4, an engineered protein cage that encapsulates its own encoding mRNA, we successfully blocked spontaneous capsid assembly, allowing isolation of the individual monomers in soluble form. To init ...[more]
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