Dataset Information
In situ cryo-electron tomograms #1-#3 of native mouse heart muscle from SOLIST procedure.
Ontology highlight
ABSTRACT:
SUBMITTER: Philipp S Erdmann
PROVIDER: EMPIAR-12123 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Serialized on-grid lift-in sectioning for tomography (SOLIST) enables a biopsy at the nanoscale.
Nature methods 20240913 9
Cryo-focused ion beam milling has substantially advanced our understanding of molecular processes by opening windows into cells. However, applying this technique to complex samples, such as tissues, has presented considerable technical challenges. Here we introduce an innovative adaptation of the cryo-lift-out technique, serialized on-grid lift-in sectioning for tomography (SOLIST), addressing these limitations. SOLIST enhances throughput, minimizes ice contamination and improves sample stabilit ...[more]