ShCas12k in complex with a sgRNA and a dsDNA target
Ontology highlight
ABSTRACT:
SUBMITTER: Martin Jinek
PROVIDER: EMPIAR-12264 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Target site selection and remodelling by type V CRISPR-transposon systems.
Querques Irma I Schmitz Michael M Oberli Seraina S Chanez Christelle C Jinek Martin M
Nature 20211110 7885
Canonical CRISPR-Cas systems provide adaptive immunity against mobile genetic elements<sup>1</sup>. However, type I-F, I-B and V-K systems have been adopted by Tn7-like transposons to direct RNA-guided transposon insertion<sup>2-7</sup>. Type V-K CRISPR-associated transposons rely on the pseudonuclease Cas12k, the transposase TnsB, the AAA+ ATPase TnsC and the zinc-finger protein TniQ<sup>7</sup>, but the molecular mechanism of RNA-directed DNA transposition has remained elusive. Here we report ...[more]
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