Dataset Information
Cryo electron tomography on xenon plasma FIB lamellae of high-pressure frozen E. coli
Ontology highlight
ABSTRACT:
SUBMITTER: Michael Grange
PROVIDER: EMPIAR-12413 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology.
Nature communications 20250307 1
Cryo focused ion beam lamella preparation is a potent tool for in situ structural biology, enabling the study of macromolecules in their native cellular environments. However, throughput is currently limited, especially for thicker, more biologically complex samples. We describe how xenon plasma focused ion beam milling can be used for routine bulk milling of thicker, high-pressure frozen samples. We demonstrate lamellae preparation with a high success rate on these samples and determine a 4.0 Å ...[more]