Dataset Information
Single Particle cryo-EM dataset of the shaped pulse revitrified 50S ribosomal subunit
Ontology highlight
ABSTRACT:
SUBMITTER: Monique Sarah Straub
PROVIDER: EMPIAR-12436 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Laser flash melting cryo-EM samples to overcome preferred orientation.
Nature methods 20250828 9
Sample preparation remains a bottleneck for protein structure determination by cryo-electron microscopy. A frequently encountered issue is that proteins adsorb to the air-water interface of the sample in a limited number of orientations. This makes it challenging to obtain high-resolution reconstructions, or may even cause projects to fail altogether. We have previously observed that laser flash melting and revitrification of cryo-EM samples reduces preferred orientation for large, symmetric par ...[more]