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Cryo electron tomography of Chlamydomonas reinhardtii prepared using FIB milling

ABSTRACT:

SUBMITTER: Xueming Li

PROVIDER: EMPIAR-12469 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

MPicker: visualizing and picking membrane proteins for cryo-electron tomography.

Yan Xiaofeng X Li Shudong S Huang Weilin W Wang Hao H Zhao Tianfang T Huang Mingtao M Zhou Niyun N Shen Yuan Y Li Xueming X

Nature communications 20250108 1

Advancements in cryo-electron tomography (cryoET) allow the structure of macromolecules to be determined in situ, which is crucial for studying membrane protein structures and their interactions in the cellular environment. However, membranes are often highly curved and have a strong contrast in cryoET tomograms, which masks the signals from membrane proteins. These factors pose difficulties in observing and revealing the structures of membrane proteins in situ. Here, we report a membrane-flatte ...[more]

PMID: 39774981

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