Dataset Information
Single-particle cryoEM data of copper-loaded ABC transporter MsbA trapped with ADP-vanadate
Ontology highlight
ABSTRACT:
SUBMITTER: Minglei Zhao
PROVIDER: EMPIAR-12504 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis for lipid and copper regulation of the ABC transporter MsbA.
Nature communications 20221126 1
A critical step in lipopolysaccharide (LPS) biogenesis involves flipping lipooligosaccharide, an LPS precursor, from the cytoplasmic to the periplasmic leaflet of the inner membrane, an operation carried out by the ATP-binding cassette transporter MsbA. Although LPS binding to the inner cavity of MsbA is well established, the selectivity of MsbA-lipid interactions at other site(s) remains poorly understood. Here we use native mass spectrometry (MS) to characterize MsbA-lipid interactions and gui ...[more]