Dataset Information
Single-particle cryoEM data of copper-loaded ABC transporter MsbA in the presence of ATP for 6 hours
Ontology highlight
ABSTRACT:
SUBMITTER: Minglei Zhao
PROVIDER: EMPIAR-12507 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Native mass spectrometry and structural studies reveal modulation of MsbA-nucleotide interactions by lipids.
Nature communications 20240715 1
The ATP-binding cassette (ABC) transporter, MsbA, plays a pivotal role in lipopolysaccharide (LPS) biogenesis by facilitating the transport of the LPS precursor lipooligosaccharide (LOS) from the cytoplasmic to the periplasmic leaflet of the inner membrane. Despite multiple studies shedding light on MsbA, the role of lipids in modulating MsbA-nucleotide interactions remains poorly understood. Here we use native mass spectrometry (MS) to investigate and resolve nucleotide and lipid binding to Msb ...[more]