Dataset Information
Motion-corrected single-particle cryo-EM dataset of PI3KC3-C1 bound to RAB1A(Q70L)
Ontology highlight
ABSTRACT:
SUBMITTER: James H Hurley
PROVIDER: EMPIAR-12526 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural pathway for PI3-kinase regulation by VPS15 in autophagy.
Science (New York, N.Y.) 20250411 6743
The class III phosphatidylinositol-3 kinase complexes I and II (PI3KC3-C1 and PI3KC3-C2) have vital roles in macroautophagy and endosomal maturation, respectively. We elucidated a structural pathway of enzyme activation through cryo-electron microscopy analysis of PI3KC3-C1. The inactive conformation of the VPS15 pseudokinase stabilizes the inactive conformation, sequestering its <i>N</i>-myristate in the N-lobe of the pseudokinase. Upon activation, the myristate is liberated such that the VPS34 ...[more]