Dataset Information
Ensemble cryo-EM reveals conformational states of the nsp13 helicase replication-transcription complex
Ontology highlight
ABSTRACT:
SUBMITTER: Seth A Darst
PROVIDER: EMPIAR-12600 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nature structural & molecular biology 20220308 3
The SARS-CoV-2 nonstructural proteins coordinate genome replication and gene expression. Structural analyses revealed the basis for coupling of the essential nsp13 helicase with the RNA-dependent RNA polymerase (RdRp) where the holo-RdRp and RNA substrate (the replication-transcription complex or RTC) associated with two copies of nsp13 (nsp13<sub>2</sub>-RTC). One copy of nsp13 interacts with the template-RNA in an opposing polarity to the RdRp and is envisaged to drive the RdRp backward on the ...[more]