Dataset Information
Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
Ontology highlight
ABSTRACT:
SUBMITTER: Ritwika Basu
PROVIDER: EMPIAR-12613 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome.
Science (New York, N.Y.) 20250619 6753
Translation termination is essential in all living organisms because it ensures that proteins have lengths strictly defined by their genes. This universal process is mediated by peptide release factors (RFs) that recognize stop codons and catalyze the hydrolysis of peptidyl transfer RNA (peptidyl-tRNA) on the ribosome, presumably by activating a water molecule. We report structures of the bacterial ribosome in complex with peptidyl-tRNA and RFs in the prepeptide release state. No hydrolytic wate ...[more]