Dataset Information
TnsB strand transfer complex micrographs
Ontology highlight
ABSTRACT:
SUBMITTER: Guillermo Montoya
PROVIDER: EMPIAR-12652 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon.
Nature communications 20221002 1
CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opted CRISPR-Cas systems for RNA-guided transposition. Here we present the 2.4 Å cryo-EM structure of the Scytonema hofmannii (sh) TnsB transposase from Type V-K CAST, bound to the strand transfer DNA. The strand transfer complex displays an intertwined pseudo-symmetrical architecture. Two protomers involved in strand transfer display a catalytically competent active site composed by DDE residues, while other two, which pl ...[more]
