Dataset Information
Mechanistic snapshots of lipid-linked sugar transfer
Ontology highlight
ABSTRACT:
SUBMITTER: Ryan Timothy Morgan
PROVIDER: EMPIAR-12683 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Mechanistic snapshots of lipid-linked sugar transfer.
Nature communications 20251206 1
Enzymes undergo dynamic conformational changes during catalysis, yet conventional high-resolution structural methods typically capture only the most stable states. Here, we address this gap using rapid UV photolysis of a chemically caged substrate with cryogenic time-resolved electron microscopy (cryo-TREM). We elucidate the catalytic mechanism of GtrB, a membrane-bound glycosyltransferase that transfers glucose from UDP-glucose to the lipid carrier undecaprenyl phosphate. We visualized how GtrB ...[more]