Dataset Information
Intermediate states of ATAD5-RFC incubated with PCNA for 20min
Ontology highlight
ABSTRACT:
SUBMITTER: Huilin Li
PROVIDER: EMPIAR-12710 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
The human ATAD5 has evolved unique structural elements to function exclusively as a PCNA unloader.
Nature structural & molecular biology 20240613 11
Humans have three different proliferating cell nuclear antigen (PCNA) clamp-loading complexes: RFC and CTF18-RFC load PCNA onto DNA, but ATAD5-RFC can only unload PCNA from DNA. The underlying structural basis of ATAD5-RFC unloading is unknown. We show here that ATAD5 has two unique locking loops that appear to tie the complex into a rigid structure, and together with a domain that plugs the DNA-binding chamber, prevent conformation changes required for DNA binding, likely explaining why ATAD5-R ...[more]