Dataset Information
CryoEM dataset of Retron-Eco1 "-1-turn" mutant filaments in the presence of NAD+
Ontology highlight
ABSTRACT:
SUBMITTER: Arturo Carabias
PROVIDER: EMPIAR-12799 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Retron-Eco1 assembles NAD<sup>+</sup>-hydrolyzing filaments that provide immunity against bacteriophages.
Molecular cell 20240523 11
Retrons are toxin-antitoxin systems protecting bacteria against bacteriophages via abortive infection. The Retron-Eco1 antitoxin is formed by a reverse transcriptase (RT) and a non-coding RNA (ncRNA)/multi-copy single-stranded DNA (msDNA) hybrid that neutralizes an uncharacterized toxic effector. Yet, the molecular mechanisms underlying phage defense remain unknown. Here, we show that the N-glycosidase effector, which belongs to the STIR superfamily, hydrolyzes NAD<sup>+</sup> during infection. ...[more]
