Dataset Information
Cryo-EM micrographs of R2Tg retrotransposon initiating target-primed reverse transcription
Ontology highlight
ABSTRACT:
SUBMITTER: Max Wilkinson
PROVIDER: EMPIAR-12960 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons.
Nature communications 20250702 1
R2 elements, a class of non-long terminal repeat (non-LTR) retrotransposons, have the potential to be harnessed for transgene insertion. However, efforts to achieve this are limited by our understanding of the retrotransposon mechanisms. Here, we structurally and biochemically characterize R2 from Taeniopygia guttata (R2Tg). We show that R2Tg cleaves both strands of its ribosomal DNA target and binds a pseudoknotted RNA element within the R2 3' UTR to initiate target-primed reverse transcription ...[more]