Dataset Information
Single particle cryo-EM dataset of as-prepared Bos taurus mitochondrial complex I (oxidised) in MSP2N2
Ontology highlight
ABSTRACT:
SUBMITTER: Judy Hirst
PROVIDER: EMPIAR-13132 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy.
Nature communications 20220519 1
Mitochondrial complex I is a central metabolic enzyme that uses the reducing potential of NADH to reduce ubiquinone-10 (Q<sub>10</sub>) and drive four protons across the inner mitochondrial membrane, powering oxidative phosphorylation. Although many complex I structures are now available, the mechanisms of Q<sub>10</sub> reduction and energy transduction remain controversial. Here, we reconstitute mammalian complex I into phospholipid nanodiscs with exogenous Q<sub>10</sub>. Using cryo-EM, we re ...[more]