Dataset Information
Structural basis for loading of Transcription Repair-Coupling factor Mfd onto stalled elongation complexes
Ontology highlight
ABSTRACT:
SUBMITTER: Seth Darst
PROVIDER: EMPIAR-13227 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Structural basis for loading of Transcription Repair-Coupling factor Mfd onto stalled elongation complexes.
bioRxiv : the preprint server for biology 20250906
Transcription-coupled repair (TCR) is a nucleotide excision repair sub-pathway that preferentially removes lesions from the DNA template-strand stalling RNA polymerase (RNAP) elongation complexes (ECs). In bacteria, the superfamily 2 Mfd translocase mediates TCR by displacing stalled ECs and recruiting Uvr(A)B. Using cryo-electron microscopy, we previously visualized seven Mfd-EC complexes spanning the ATP -dependent Mfd loading and EC displacement pathway [L1->L2(ADP)->C1(ATP)->C2(ATP)->C3(ADP) ...[more]
