Dataset Information
Cryo-EM structure of the desthiobiotin-bound streptavidin prepared using the cryoWriter automated grid preparation system with line pattern writing
Ontology highlight
ABSTRACT:
SUBMITTER: Chinmaya KV
PROVIDER: EMPIAR-13336 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
CryoWriter: a robotic solution for improved Cryo-EM grid preparation.
Nature communications 20260530 1
Cryo-electron microscopy (cryo-EM) structure determination relies on preparing thin, vitreous films of sample solution on EM grids. Cryo-EM is a mature technology, but preparing the grids remains a major bottleneck. Here, we evaluate the cryoWriter, a blotting-free, microfluidic grid-preparation robot that writes nanoliter volumes onto EM grids in a controlled environment. Using capillary-writing in spiral or line patterns, we prepared high-quality grids from minimal sample volumes and obtained ...[more]
