Dataset Information
Human Hemoglobin, Laser Phase Plate TEM, Laser-ON dataset 2 (H2)
Ontology highlight
ABSTRACT:
SUBMITTER: Holger Mueller
PROVIDER: EMPIAR-13534 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Laser phase plate improves structure determination of small proteins by cryo-EM.
Science (New York, N.Y.) 20260611 6807
Phase plates can, in principle, overcome the poor image contrast in cryo-electron microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous designs have been unstable and compromised the high-resolution signal and have thus been unable to surpass results achieved by standard cryo-EM. Here, we show that the laser phase plate (LPP), installed in a modern, custom Titan Krios microscope, enhances the resolution in single-particle reconstructi ...[more]