Dataset Information
Single Particle Cryo-EM Structure of Thermotoga maritima Threonylcarbamoyl Transfer Complex (TsaB2D) in Complex with Thermotoga maritima tRNA(LYS)
Ontology highlight
ABSTRACT:
SUBMITTER: Ana Kutchuashvili
PROVIDER: EMPIAR-13543 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Anticodon loop remodeling and D-stem shape drive the specific recognition of ANN-decoding tRNAs for t6A modification.
Nucleic acids research 20260601 11
N6-threonylcarbamoyladenosine (t6A) is a universal transfer RNA (tRNA) modification essential for translational fidelity. The modification is installed at position 37 of ANN-decoding tRNAs (N is A, U, G, or C) by transfer of a threonylcarbamoyl moiety from a pathway intermediate, catalyzed in bacteria by the TsaBD complex. Despite the strict requirement for the 36-UAA-38 sequence in substrate tRNAs, the structural basis for this specificity has remained unclear. We determined cryo-EM structures ...[more]