Dataset Information
In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles
Ontology highlight
ABSTRACT:
SUBMITTER: Andrei A Korostelev
PROVIDER: EMPIAR-13577 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
<i>In extracto</i> cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles.
bioRxiv : the preprint server for biology 20251125
Cryogenic electron microscopy (cryo-EM) made impressive progress in resolving cellular macromolecules and their detailed interactions. Single-particle cryo-EM traditionally relies on purified macromolecules and lacks the complexity of cellular environments, whereas <i>in situ</i> cryo-EM or cryo-ET require extensive sample preparation and data acquisition, presenting challenges in achieving high resolution. We describe cryo-EM of cellular lysates- <i>in extracto</i> cryo-EM-allowing the flexibil ...[more]