Dataset Information
Cryo-EM single particle micrographs of hypomethylated 80S ribosomes
Ontology highlight
ABSTRACT:
SUBMITTER: Hong Li
PROVIDER: EMPIAR-13642 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
2'-O-Methylation maintains ribosome structural and translation integrity.
Molecular cell 20260401 8
In eukaryotic ribosomes, ∼2% of RNA nucleotides undergo 2'-O-methylation, a conserved cellular mechanism thought to be critical for maintaining and regulating translation. Here, we use ribosome profiling, translation assays, proteomics, and high-resolution structural analyses to show that loss of native 2'-O-methylation in yeast ribosomes disproportionally affects the translation of ribosomal protein transcripts, driven by changes in codon usage and recognition of structured RNA. Translation rep ...[more]