Dataset Information
Single-Particle Cryo-EM of a Nucleosome Containing a Single-Strand Break at SHL −2.8 Bound to the Human PARP2
Ontology highlight
ABSTRACT:
SUBMITTER: Tae Hun Kim
PROVIDER: EMPIAR-13698 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
High-Yield Production of Modified DNA Enables Structural Analysis of PARP2 Recognition of Nucleosomal Single-Strand Breaks.
Journal of molecular biology 20260310 10
Preparation of high-quality nucleosomal DNA substrates in milligram quantities remains a major bottleneck for mechanistic studies of chromatin-associated processes. Here, we present an optimized large-scale PCR workflow that enables rapid, low-cost production of diverse nucleosomal DNAs suitable for biochemical assays and high-resolution cryo-EM. Systematic optimization of amplification conditions yields milligram quantities of homogeneous DNA that can be fluorescently or biotin-labeled and enzy ...[more]
