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RaynaNolen_2024_PFOS_Zebrafish_GEMs

ABSTRACT: Condition specific zebrafish metabolic models generated using the COBRA MetaboTools framework. The Wang et al., (2021) zebrafish genome-scale metabolic model (GEM) was constrained with experimental data from 5 days post fertilized (dpf) zebrafish to generate a 'base-model'. In turn this 5 dpf zebrafish base-model was constrained with experimental (transcriptomics and metabolomics) data from 5 dpf zebrafish exposed to the environmental pollutant perfluorooctane sulfonate (PFOS), at three levels - Low (0.06 uM), Medium (0.6 uM), and High (2 uM) PFOS.The MetaboTools framework was used to construct three condition-sepcific models: Low, Medium, and High PFOS.Key simulation predictions of effects on the carnitine shuttle and lipid metabolism were confirmed in wild-caught fish and dolphins (stranded animals) sampled from the northern Gulf of Mexico - published in Nolen et al., (2024) https://doi.org/10.1016/j.cbpc.2023.109817

SUBMITTER: David Hala

PROVIDER: MODEL2403010004 | biostudies-other |

SECONDARY ACCESSION(S): 10.3389/fsysb.2024.1367562

REPOSITORIES: biostudies-other

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