The IFT81-IFT74 complex enhances GTP hydrolysis to inactivate RabL2 during intraflagellar transport

ABSTRACT: Cilia are important cellular organelles for signaling and motility and are constructed via intraflagellar transport (IFT). RabL2 is a small GTPase that localizes to the basal body of cilia via an interaction with the centriolar protein CEP19 before downstream association with the IFT machinery, which is followed by initiation of IFT. We reconstituted and purified RabL2 with CEP19 or IFT proteins to show that a reconstituted pentameric IFT complex containing IFT81/74 enhances the GTP hydrolysis rate in RabL2. The binding site on IFT81/74 that promotes GTP hydrolysis in RabL2 was mapped to a 70 amino acid long coiled-coil region of IFT81/74. We present structural models for RabL2-containing IFT complexes that we validate in vitro and in cellulo and demonstrate that Chlamydomonas IFT81/74 en

ORGANISM(S): Homo sapiens (human) Chlamydomonas reinhardtii

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PROVIDER: S-BSST1153 | biostudies-other |

REPOSITORIES: biostudies-other