Chromosome genomics facilitates the marker development and selection of wheat-Aegilops biuncialis addition, substitution and translocation lines

ABSTRACT: The annual goatgrass, Aegilops biuncialis is a rich source of genes with considerable agronomic value. This genetic potential can be exploited for wheat improvement through interspecific hybridization to increase stress resistance, grain quality and adaptability. However, development of alien introgressions is hampered by the low-throughput of cytogenetic selection. Using sequence of flow-sorted chromosomes of the diploid progenitors, the present study allowed the development of chromosome-specific markers. In total, 482 PCR markers were validated on wheat (Mv9kr1) and Ae. biuncialis (MvGB642) crossing partners and 126 on wheat-Aegilops additions. Thirthy-two markers specific for U- or M- chromosomes were used in combination with GISH and FISH for screening 44 Mv9kr1 x Ae. biuncialis BC3

ORGANISM(S): Aegilops Aegilops biuncialis Triticum aestivum (wheat)

SUBMITTER:  

PROVIDER: S-BSST1163 | biostudies-other |

REPOSITORIES: biostudies-other