Interferon Regulatory Factor 4 plays a pivotal role in the development of aGVHD-associated colitis

ABSTRACT: These RNA Seq data derive from re-isolated WT or IRF4 knockout CD4+ T cells 15 days after transplantation in a C57BL/6 → BALB/c acute GVHD model. In brief, BALB/c recipient mice were lethally irradiated with 8Gy at day 0. CD90.2 depleted bone marrow cells were isolated from C57BL/6 donors, and transplanted with a concentration of 5x10^6 bone marrow cells per recipient at day 1. CD4+ T cells were isolated from spleens of F2- and F3-generation C57BL/6 IRF4-/- CD45.1-Luc+ (Irf4 -/-) or wild type C57BL/6 IRF4 +/+ CD45.1-Luc+ (WT) mice and transplanted with a concentration of 1x10^6 cells per BALB/c recipient. At day 15, CD4+ T cells were re-isolated from the host spleens via CD4+ T cell isolation Kit II (Miltenyi) and FACS sorting for NKp46 negative, CD8 negative CD3 positive CD4 positive CD4

ORGANISM(S): Mus musculus (mouse)

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PROVIDER: S-BSST1167 | biostudies-other |

REPOSITORIES: biostudies-other