Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types.

ABSTRACT: The description of precise miRNA expression patterns is crucial to understand what these small RNAs contribute to animal development and physiology. High-throughput sequencing of miRNAs from individual developmental stages has provided insight into temporal regulation but often lacks the cellular resolution to link miRNA-function to the biology of distinct cell types within complex tissues. Mime-seq 2.0 takes advantage of an engineered methyltransferase to methylate miRNAs in vivo in mice in cell types of interest. This chemical small RNA-tagging approach is followed by chemo-selective, high-throughput sequencing that enables the identification of tissue- and cell type-specific miRNA profiles in a sensitive and reproducible manner. Flow cytometry data produced as part of this study to inve

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:  

PROVIDER: S-BSST1360 | biostudies-other |

REPOSITORIES: biostudies-other