Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation

ABSTRACT: The multifunctional RNA-binding protein hnRNPL has been implicated in antibody class switching but its broader function in B cells is unknown. Here, we show that hnRNPL is essential for B cell activation, germinal center, and antibody responses. Upon activation, hnRNPL-deficient B cells show proliferation defects and increased apoptosis. Comparative analysis of RNA-seq data from activated B cells and another 8 hnRNPL-depleted cell types reveals a common effect on the MYC and E2F transcriptional programs required for proliferation. Notably, while individual gene expression changes were cell type specific, several alternative splicing events affecting histone modifiers like KDM6A and SIRT1, were conserved across cell types. Moreover, hnRNPL-deficient B cells showed global changes in H3K27me3

ORGANISM(S): Mus musculus (mouse)

SUBMITTER:  

PROVIDER: S-BSST1365 | biostudies-other |

SECONDARY ACCESSION(S): GSE242069

REPOSITORIES: biostudies-other